Hotspot

ndhF

Genome · 105261-107507 · 2247 bp

Back to Phegopteris

Pi 0.0685

MarkerSeek score 75.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2247
variable_sites: 154
pi: 0.0685
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCCCATTATAGGAGGAGGCG	CCGCCGATGTCAATTATTGC	55.0	50.0	58.1	58.0	2936 bp	1.0	85.0
ndhF_p2	AGTAAACTTCGCGTACACCC	CCGCCGATGTCAATTATTGC	50.0	50.0	57.9	58.0	2960 bp	1.0	84.9
ndhF_p3	GTAAACTTCGCGTACACCCT	CCGCCGATGTCAATTATTGC	50.0	50.0	57.9	58.0	2959 bp	1.0	84.9
ndhF_p4	AACTTCGCGTACACCCTAAC	CCGCCGATGTCAATTATTGC	50.0	50.0	57.9	58.0	2956 bp	0.5	59.1
ndhF_p5	CCCCTCCAGGAAGCATAGTA	TTCTGCCTTTAACAGCTGCA	55.0	45.0	57.9	58.0	2947 bp	0.5	59.0