Hotspot

trnT-UGU-trnR-ACG

Genome · 84021-84673 · 653 bp

Back to Phegopteris

Pi 0.0611

MarkerSeek score 82.40

Primer available yes

Label trnT-UGU-trnR-ACG

Feature Metadata

label_name: trnT-UGU-trnR-ACG
feature_type: igs
region: Genome
length_bp: 653
variable_sites: 39
pi: 0.0611
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnR-ACG_p1	AGTACGGAGGTTCGAATCCT	CTCAGGGGATTAGAGCACGT	50.0	55.0	57.9	58.9	749-827 bp	1.0	77.3
trnT-UGU-trnR-ACG_p2	AGTACGGAGGTTCGAATCCT	TCAGGGGATTAGAGCACGTG	50.0	55.0	57.9	59.2	748-826 bp	1.0	75.0
trnT-UGU-trnR-ACG_p3	AGTACGGAGGTTCGAATCCT	TCAGGGGATTAGAGCACGT	50.0	52.6	57.9	57.7	748-826 bp	1.0	74.0
trnT-UGU-trnR-ACG_p4	AGTACGGAGGTTCGAATCCT	CAGGGGATTAGAGCACGTG	50.0	57.9	57.9	57.6	747-825 bp	1.0	73.4
trnT-UGU-trnR-ACG_p5	CGTAGTACGGAGGTTCGAATC	CTCAGGGGATTAGAGCACGT	52.4	55.0	58.0	58.9	752-830 bp	1.0	70.2