Hotspot

petA-psbJ

Genome · 59240-60091 · 852 bp

Back to Phegopteris

Pi 0.0777
MarkerSeek score 82.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
Genome
length_bp
852
variable_sites
65
pi
0.0777
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GTTTGAAAAAGTGCAGTTGGC GCCGGTACACTTGTGATAGG 42.9 55.0 57.3 58.1 970-988 bp 1.0 72.8
petA-psbJ_p2 GTTTGAAAAAGTGCAGTTGGC TTCGCTAGGCATATCCCCTT 42.9 50.0 57.3 58.3 950-968 bp 1.0 71.3
petA-psbJ_p3 GTTTGAAAAAGTGCAGTTGGC CTGTAGCCGGTACACTTGTG 42.9 55.0 57.3 58.3 975-993 bp 1.0 70.9
petA-psbJ_p4 GTTTGAAAAAGTGCAGTTGGC CCCTGTGGTTGGTAGGTACT 42.9 55.0 57.3 58.3 993-1011 bp 1.0 70.4
petA-psbJ_p5 GTTTGAAAAAGTGCAGTTGGC GGTAGGTACTGTAGCCGGTA 42.9 55.0 57.3 57.7 983-1001 bp 1.0 70.4