Hotspot

ndhC-trnV-UAC

Genome · 47797-48312 · 516 bp

Back to Phegopteris

Pi 0.103
MarkerSeek score 80.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
Genome
length_bp
516
variable_sites
52
pi
0.103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTGGTGGAGCAGAAACA TCGAATCCGTATAGCCCTA 40.9 47.4 57.7 54.3 553-558 bp 1.0 48.9
ndhC-trnV-UAC_p2 CATATTGGTGGAGCAGAAACA TCGAATCCGTATAGCCCTA 42.9 47.4 56.2 54.3 552-557 bp 1.0 48.9
ndhC-trnV-UAC_p3 TCATATTGGTGGAGCAGAAAC TCGAATCCGTATAGCCCTA 42.9 47.4 55.9 54.3 553-558 bp 1.0 48.9
ndhC-trnV-UAC_p4 TCATATTGGTGGAGCAGAAACAT TCGAATCCGTATAGCCCTA 39.1 47.4 58.1 54.3 553-558 bp 1.0 48.9
ndhC-trnV-UAC_p5 CATATTGGTGGAGCAGAAACAT TCGAATCCGTATAGCCCTA 40.9 47.4 56.7 54.3 552-557 bp 1.0 48.9