Hotspot

trnL-UAA-trnF-GAA

Genome · 45497-45818 · 322 bp

Back to Phegopteris

Pi 0.103

MarkerSeek score 79.90

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: Genome
length_bp: 322
variable_sites: 32
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	ATCCGTTGGTTTCACAGGAC	TTTGAACTGGTGACACGAGG	50.0	50.0	58.1	58.1	419-429 bp	1.0	86.6
trnL-UAA-trnF-GAA_p2	ATCCGTTGGTTTCACAGGAC	TGCCAAGAACCAGGTTTGAA	50.0	45.0	58.1	57.8	433-443 bp	1.0	85.6
trnL-UAA-trnF-GAA_p3	ATCCGTTGGTTTCACAGGAC	AGGTTTGAACTGGTGACACG	50.0	50.0	58.1	58.3	422-432 bp	1.0	84.3
trnL-UAA-trnF-GAA_p4	ATCCGTTGGTTTCACAGGAC	GGTTTGAACTGGTGACACGA	50.0	50.0	58.1	58.3	421-431 bp	1.0	84.3
trnL-UAA-trnF-GAA_p5	ATCCGTTGGTTTCACAGGAC	AATACCAGAACTGCTTCGGC	50.0	50.0	58.1	58.3	474-484 bp	1.0	84.1