Hotspot

trnT-GGU-trnfM-CAU

Genome · 35067-35543 · 477 bp

Back to Phegopteris

Pi 0.108

MarkerSeek score 85.70

Primer available yes

Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name: trnT-GGU-trnfM-CAU
feature_type: igs
region: Genome
length_bp: 477
variable_sites: 47
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnfM-CAU_p1	GGTTAGCCCTTTGTCGGATT	GTTCAAATCCTGTCTCCGCA	50.0	50.0	57.9	58.2	550-574 bp	1.0	88.2
trnT-GGU-trnfM-CAU_p2	GGTTAGCCCTTTGTCGGATT	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.9	57.8	584-608 bp	1.0	87.5
trnT-GGU-trnfM-CAU_p3	GGTTAGCCCTTTGTCGGATT	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.9	57.8	583-607 bp	1.0	87.5
trnT-GGU-trnfM-CAU_p4	AGCCCTTTGTCGGATTTGAA	GTTCAAATCCTGTCTCCGCA	45.0	50.0	57.7	58.2	546-570 bp	1.0	86.9
trnT-GGU-trnfM-CAU_p5	AGCCCTTTGTCGGATTTGAA	TAGCTCGCAAGGCTCATAAC	45.0	50.0	57.7	57.8	579-603 bp	1.0	86.3