Hotspot

psbZ-trnS-UGA

Genome · 30485-30916 · 432 bp

Back to Phegopteris

Pi 0.115

MarkerSeek score 79.70

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: Genome
length_bp: 432
variable_sites: 45
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	TTTCTACGGGATAGGCGGAT	CGATACCGAACCCGTTCTTT	50.0	50.0	58.0	58.0	1347-1372 bp	1.0	89.1
psbZ-trnS-UGA_p2	TTTCTACGGGATAGGCGGAT	AGATCAACGCCGTGAACTAC	50.0	50.0	58.0	58.0	1492-1517 bp	1.0	88.3
psbZ-trnS-UGA_p3	TTGCCCCTCCTTTTTCCTTC	CGATACCGAACCCGTTCTTT	50.0	50.0	58.0	58.0	1501 bp	0.5	59.9
psbZ-trnS-UGA_p4	CTTGCCCCTCCTTTTTCCTT	CGATACCGAACCCGTTCTTT	50.0	50.0	58.0	58.0	1502 bp	0.5	59.9
psbZ-trnS-UGA_p5	TTGCCCCTCCTTTTTCCTTC	AGATCAACGCCGTGAACTAC	50.0	50.0	58.0	58.0	1646 bp	0.5	59.9