Hotspot

trnC-GCA-trnG-GCC

Genome · 29537-29976 · 440 bp

Back to Phegopteris

Pi 0.118

MarkerSeek score 81.50

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: Genome
length_bp: 440
variable_sites: 46
pi: 0.118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	GATTTGCAGTCCTCCGTCTT	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	472-500 bp	1.0	81.3
trnC-GCA-trnG-GCC_p2	GATTTGCAGTCCTCCGTCTT	GGTTCGATTCCCGCTATCC	50.0	57.9	57.9	57.8	473-501 bp	1.0	81.1
trnC-GCA-trnG-GCC_p3	TTAGGCGACACCCAGATTTG	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	507-535 bp	1.0	80.7
trnC-GCA-trnG-GCC_p4	GTTAGGCGACACCCAGATTT	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	508-536 bp	1.0	80.7
trnC-GCA-trnG-GCC_p5	TTTCTTGTTAGGCGACACCC	GTTCGATTCCCGCTATCCG	50.0	57.9	58.1	58.2	514-542 bp	1.0	80.5