Hotspot

trnS-GCU-ycf12

Genome · 7997-8556 · 560 bp

Back to Phegopteris

Pi 0.12

MarkerSeek score 78.20

Primer available yes

Label trnS-GCU-ycf12

Feature Metadata

label_name: trnS-GCU-ycf12
feature_type: igs
region: Genome
length_bp: 560
variable_sites: 67
pi: 0.12
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-ycf12_p1	TAATGTCTCACGAAAGCCGG	TTACCAACGCCAAGATAGCC	50.0	50.0	58.0	58.0	1040 bp	0.5	59.7
trnS-GCU-ycf12_p2	TAATGTCTCACGAAAGCCGG	GAATGGCGCATTCTTTTCCC	50.0	50.0	58.0	58.1	1438 bp	0.5	59.4
trnS-GCU-ycf12_p3	TAATGTCTCACGAAAGCCGG	GGAATGGCGCATTCTTTTCC	50.0	50.0	58.0	58.1	1439 bp	0.5	59.4
trnS-GCU-ycf12_p4	GGGAGAGAGAGGGATTCGAA	TTACCAACGCCAAGATAGCC	55.0	50.0	57.9	58.0	694 bp	0.5	59.1
trnS-GCU-ycf12_p5	AAGGGAGAGAGAGGGATTCG	TTACCAACGCCAAGATAGCC	55.0	50.0	57.9	58.0	696 bp	0.5	59.1