Hotspot

ndhF

SSC · 109041-111266 · 2226 bp

Back to Phedimus

Pi 0.018

MarkerSeek score 53.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 141
pi: 0.018
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGACGTCCGTTGCGATAT	TTAACGCTGCCCAATATCCC	52.6	50.0	57.5	58.0	2829-2852 bp	1.0	75.2
ndhF_p2	TGGGACTCAAGAAACTTCGA	TTAACGCTGCCCAATATCCC	45.0	50.0	56.4	58.0	2933-2966 bp	1.0	75.0
ndhF_p3	GAGACGTCCGTTGCGATAT	AAAAGGCTTTTAACGCTGCC	52.6	45.0	57.5	57.9	2838-2861 bp	1.0	74.2
ndhF_p4	TGGGACTCAAGAAACTTCGA	AAAAGGCTTTTAACGCTGCC	45.0	45.0	56.4	57.9	2942-2975 bp	1.0	74.1
ndhF_p5	GAGACGTCCGTTGCGATAT	TACGCTTTTTGGCCAGAGAA	52.6	45.0	57.5	57.7	2784-2807 bp	0.923077	68.6