Hotspot

ndhF-rpl32

SSC · 111267-111771 · 505 bp

Back to Phedimus

Pi 0.0313

MarkerSeek score 67.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 505
variable_sites: 55
pi: 0.0313
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCAAAATCCCCGATACGGTT	TTAACGCTGCCCAATATCCC	50.0	50.0	58.0	58.0	1152-1170 bp	1.0	88.5
ndhF-rpl32_p2	CCAAAATCCCCGATACGGTT	TTCGAACCGAGATGCTCAAG	50.0	50.0	58.0	58.0	1752-1780 bp	1.0	88.4
ndhF-rpl32_p3	CCAAAATCCCCGATACGGTT	AAGCACTGCTTCCTAAGAGC	50.0	50.0	58.0	57.9	1735-1763 bp	1.0	87.7
ndhF-rpl32_p4	CCAAAATCCCCGATACGGTT	AAAGGGAGAGATGGGGGAAA	50.0	50.0	58.0	57.9	1491-1501 bp	0.769231	71.5
ndhF-rpl32_p5	CCAAAATCCCCGATACGGTT	TTGTGCGAAAGGGAGAGATG	50.0	50.0	58.0	57.9	1498-1508 bp	0.769231	71.3