Hotspot

ndhC-trnV-UAC

LSC · 49910-50789 · 880 bp

Back to Phedimus

Pi 0.0208

MarkerSeek score 63.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 61
pi: 0.0208
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGAAATGCCCAGAAAATATCA	AGAAGGTCTACGGTTCGAGT	33.3	50.0	53.1	57.8	860-957 bp	1.0	49.2
ndhC-trnV-UAC_p2	AGAAATGCCCAGAAAATATCAT	AGAAGGTCTACGGTTCGAGT	31.8	50.0	53.8	57.8	860-957 bp	1.0	49.2
ndhC-trnV-UAC_p3	AGAAATGCCCAGAAAATATCA	GAAGGTCTACGGTTCGAGTC	33.3	55.0	53.1	57.5	859-956 bp	1.0	49.2
ndhC-trnV-UAC_p4	AGAAATGCCCAGAAAATATCA	GTTCGAGTCCGTATAGCCCT	33.3	55.0	53.1	58.7	848-945 bp	1.0	49.2
ndhC-trnV-UAC_p5	GCTTGATATTATTAGAAATGCCCAGA	AGAAGGTCTACGGTTCGAGT	34.6	50.0	57.7	57.8	873-970 bp	1.0	49.1