Hotspot

trnC-GCA-petN

LSC · 28228-29068 · 841 bp

Back to Phedimus

Pi 0.0222

MarkerSeek score 64.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 841
variable_sites: 69
pi: 0.0222
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	645-904 bp	1.0	89.8
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	639-898 bp	1.0	88.3
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	663-922 bp	1.0	87.5
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.4	57.8	657-916 bp	1.0	86.0
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TTAGAGTCCACTTCGTCCCC	50.0	55.0	58.1	58.4	701-960 bp	1.0	85.9