Hotspot

rpoB-trnC-GCA

LSC · 27021-28156 · 1136 bp

Back to Phedimus

Pi 0.0258

MarkerSeek score 66.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1136
variable_sites: 102
pi: 0.0258
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCATACACAGCAGCTCGTTC	ATTGCTTGGCTCGAGGTTAG	50.0	50.0	58.0	58.0	1588-1868 bp	0.923077	85.1
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	CTCAACAGTTCGTTTGCGTC	50.0	50.0	58.0	58.0	1803-1824 bp	0.846154	78.6
rpoB-trnC-GCA_p3	TCATACACAGCAGCTCGTTC	CTCAACAGTTCGTTTGCGTC	50.0	50.0	58.0	58.0	1508-1527 bp	0.769231	72.3
rpoB-trnC-GCA_p4	TCATACACAGCAGCTCGTTC	GTCTCAACAGTTCGTTTGCG	50.0	50.0	58.0	58.0	1510-1529 bp	0.769231	72.3
rpoB-trnC-GCA_p5	CGAGCCCATATCCTTGCTTT	GTCTCAACAGTTCGTTTGCG	50.0	50.0	58.0	58.0	1807-1826 bp	0.769231	71.9