Hotspot

rps2-rpoC2

LSC · 16395-16647 · 253 bp

Back to Phedimus

Pi 0.0324

MarkerSeek score 63.20

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 253
variable_sites: 22
pi: 0.0324
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AATGAACTCCCGCTTCCATC	AGGTTTATCGATCCCAGGGT	50.0	50.0	58.0	57.8	744-858 bp	1.0	86.6
rps2-rpoC2_p2	ACATCGAGCCCTTATTGCAG	AGGTTTATCGATCCCAGGGT	50.0	50.0	58.0	57.8	965-1079 bp	1.0	86.5
rps2-rpoC2_p3	GACATCGAGCCCTTATTGCA	AGGTTTATCGATCCCAGGGT	50.0	50.0	58.0	57.8	966-1080 bp	1.0	86.5
rps2-rpoC2_p4	AAGGTGCCATTCTCGGATTC	AGGTTTATCGATCCCAGGGT	50.0	50.0	58.0	57.8	786-900 bp	0.846154	79.3
rps2-rpoC2_p5	GCCATTCTCGGATTCCACTT	AGGTTTATCGATCCCAGGGT	50.0	50.0	58.0	57.8	781-895 bp	0.846154	79.2