Hotspot

trnS-GCU-trnG-GCC

LSC · 8759-9387 · 629 bp

Back to Phedimus

Pi 0.0347

MarkerSeek score 71.20

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 629
variable_sites: 75
pi: 0.0347
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ACCACTAAACTATACCCGCT	50.0	45.0	58.0	55.6	578-717 bp	1.0	73.1
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	ACCACTAAACTATACCCGCT	50.0	45.0	58.0	55.6	577-716 bp	1.0	73.1
trnS-GCU-trnG-GCC_p3	GCTCGTACAACGGATTAGCA	ACCACTAAACTATACCCGCT	50.0	45.0	58.1	55.6	596-735 bp	1.0	72.5
trnS-GCU-trnG-GCC_p4	ACGGAAAGAGAGGGATTCGA	ACCACTAAACTATACCCGCT	50.0	45.0	58.2	55.6	632-771 bp	1.0	72.0
trnS-GCU-trnG-GCC_p5	AGCTCGTACAACGGATTAGC	ACCACTAAACTATACCCGCT	50.0	45.0	57.8	55.6	597-736 bp	1.0	71.5