Hotspot

trnK-UUU-rps16

LSC · 4187-4841 · 655 bp

Back to Phedimus

Pi 0.0249
MarkerSeek score 63.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
655
variable_sites
57
pi
0.0249
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA CCCAATGAGCCGTTTATCGA 50.0 50.0 58.0 58.1 924-986 bp 1.0 88.7
trnK-UUU-rps16_p2 CGAATCCCTTGCTTCATCCA TCCCAATGAGCCGTTTATCG 50.0 50.0 58.0 58.1 925-987 bp 1.0 88.7
trnK-UUU-rps16_p3 ACGAATCCCTTGCTTCATCC CCCAATGAGCCGTTTATCGA 50.0 50.0 58.0 58.1 925-987 bp 1.0 88.7
trnK-UUU-rps16_p4 ACGAATCCCTTGCTTCATCC TCCCAATGAGCCGTTTATCG 50.0 50.0 58.0 58.1 926-988 bp 1.0 88.7
trnK-UUU-rps16_p5 GGACGAATCCCTTGCTTCAT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.0 58.1 927-989 bp 1.0 88.7