Hotspot

ycf1

SSC · 125320-130812 · 5493 bp

Back to Phebalium

Pi 0.00746

MarkerSeek score 43.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5493
variable_sites: 92
pi: 0.00746
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCATACTGAAACGACTCCCA	TCGAAGAATCAAAACGGTATGGA	50.0	39.1	56.9	58.2	214 bp	1.0	49.5
ycf1_p2	ACCATACTGAAACGACTCCC	TCGAAGAATCAAAACGGTATGGA	50.0	39.1	56.9	58.2	215 bp	1.0	49.5
ycf1_p3	CCATACTGAAACGACTCCCA	CGAAGAATCAAAACGGTATGGA	50.0	40.9	56.9	56.8	213 bp	1.0	49.4
ycf1_p4	CCATACTGAAACGACTCCCA	TCGAAGAATCAAAACGGTATGG	50.0	40.9	56.9	56.8	214 bp	1.0	49.4
ycf1_p5	ACCATACTGAAACGACTCCC	CGAAGAATCAAAACGGTATGGA	50.0	40.9	56.9	56.8	214 bp	1.0	49.3