Hotspot

rps16

LSC · 4648-5819 · 1172 bp

Back to Phebalium

Pi 0.00739

MarkerSeek score 61.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1172
variable_sites: 17
pi: 0.00739
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACTCCTCCACACCCTCTTTT	TCCCTCCATCTACAAGGAAA	50.0	45.0	58.2	55.1	1299-1312 bp	1.0	57.7
rps16_p2	ACTCCTCCACACCCTCTTTT	TTCCCTCCATCTACAAGGAA	50.0	45.0	58.2	55.1	1300-1313 bp	1.0	57.7
rps16_p3	TAAGACTCCTCCACACCCTC	TCCCTCCATCTACAAGGAAA	55.0	45.0	57.8	55.1	1303-1316 bp	1.0	57.3
rps16_p4	TAAGACTCCTCCACACCCTC	TTCCCTCCATCTACAAGGAA	55.0	45.0	57.8	55.1	1304-1317 bp	1.0	57.3
rps16_p5	ACTCCTCCACACCCTCTTTT	TCCCTCCATCTACAAGGAAAA	50.0	42.9	58.2	56.0	1299-1312 bp	1.0	56.4