Hotspot

ndhH-rps15

SSC · 124580-124680 · 101 bp

Back to Phebalium

Pi 0.0187

MarkerSeek score 62.40

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 101
variable_sites: 3
pi: 0.0187
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	TAAGACGAAGAACACCGTGC	CGCCAACGTCTACTAGTGTA	50.0	50.0	58.2	56.8	278-282 bp	1.0	70.7
ndhH-rps15_p2	CATTGATGGGTGGTGAGGTC	CGCCAACGTCTACTAGTGTA	55.0	50.0	58.2	56.8	259-263 bp	1.0	70.7
ndhH-rps15_p3	GGTGGTGAGGTCCCATATTG	CGCCAACGTCTACTAGTGTA	55.0	50.0	57.7	56.8	251-255 bp	1.0	69.9
ndhH-rps15_p4	TAAGACGAAGAACACCGTGC	ATTTTGGAAAAACGCCAACG	50.0	40.0	58.2	56.3	290-294 bp	1.0	66.8
ndhH-rps15_p5	TAAGACGAAGAACACCGTGC	CGCCAACGTCTACTAGTGTAT	50.0	47.6	58.2	57.3	278-282 bp	1.0	66.7