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psbE-petL

LSC · 65829-67211 · 1383 bp

Back to Phebalium

Pi 0.00564

MarkerSeek score 61.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1383
variable_sites: 18
pi: 0.00564
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCTGCTAGGAGAAAACCA	50.0	45.0	57.9	56.7	1249-1536 bp	1.0	74.5
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCTGCTAGGAGAAAACCAA	50.0	45.0	57.9	56.7	1248-1535 bp	1.0	74.5
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCTGCTAGGAGAAAACCA	50.0	45.0	57.8	56.7	1172-1459 bp	1.0	73.8
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGGAGAAAACCAA	50.0	45.0	57.8	56.7	1171-1458 bp	1.0	73.8
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCTGCTAGGAGAAAACCAA	45.0	45.0	58.2	56.7	1257-1544 bp	1.0	73.6