Hotspot

petA-psbJ

LSC · 63977-65043 · 1067 bp

Back to Phebalium

Pi 0.0114

MarkerSeek score 67.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1067
variable_sites: 29
pi: 0.0114
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	CCGGATTAGGTTCGTCCTTG	50.0	55.0	58.0	58.1	1207-1210 bp	1.0	81.2
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	TCCGGATTAGGTTCGTCCTT	50.0	50.0	58.0	58.1	1208-1211 bp	1.0	80.6
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	CCGGATTAGGTTCGTCCTTG	50.0	55.0	58.2	58.1	1204-1207 bp	1.0	80.1
petA-psbJ_p4	GCATCTGTTGTTTTGGCACA	CCGGATTAGGTTCGTCCTTG	45.0	55.0	57.8	58.1	1172-1175 bp	1.0	79.8
petA-psbJ_p5	TTGGCATCTGTTGTTTTGGC	CCGGATTAGGTTCGTCCTTG	45.0	55.0	57.8	58.1	1175-1178 bp	1.0	79.7