Hotspot

trnF-GAA-ndhJ

LSC · 49794-50264 · 471 bp

Back to Phebalium

Pi 0.0134

MarkerSeek score 53.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 14
pi: 0.0134
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATGAAATACAAGATGCATATTGA	50.0	26.1	58.1	52.3	527-530 bp	1.0	42.7
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	ATGAAATACAAGATGCATATTGA	55.0	26.1	58.0	52.3	563-566 bp	1.0	42.6
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATGAAATACAAGATGCATATTGA	55.0	26.1	57.9	52.3	551-554 bp	1.0	42.6
trnF-GAA-ndhJ_p4	AGGATGGTGTGTCGGAAATG	ATGAAATACAAGATGCATATTGA	50.0	26.1	57.9	52.3	586-589 bp	1.0	42.5
trnF-GAA-ndhJ_p5	GTCGGAAATGGTCGGGATAG	ATGAAATACAAGATGCATATTGA	55.0	26.1	57.8	52.3	576-579 bp	1.0	42.5