Hotspot

psaA-ycf3

LSC · 43254-44003 · 750 bp

Back to Phebalium

Pi 0.0081

MarkerSeek score 60.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 13
pi: 0.0081
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGGTTCTTGAGAAATGGCCC	AGCGCAGAATTGGTTGAAGA	50.0	45.0	58.1	58.1	872-896 bp	1.0	82.1
psaA-ycf3_p2	TGGTTCTTGAGAAATGGCCC	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.1	57.9	864-888 bp	1.0	81.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	846-870 bp	1.0	81.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	838-862 bp	1.0	81.7
psaA-ycf3_p5	TGGTTCTTGAGAAATGGCCC	AGCTATTGCGCTTACTCCTG	50.0	50.0	58.1	57.8	905-929 bp	1.0	80.9