Hotspot

accD

LSC · 57764-59230 · 1467 bp

Back to Phaseolus

Pi 0.0469

MarkerSeek score 74.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1467
variable_sites: 66
pi: 0.0469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ACTCCCCGCTCTCTTGTTAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	2218-2264 bp	1.0	83.9
accD_p2	ACTCCCCGCTCTCTTGTTAT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.1	57.9	2224-2270 bp	1.0	83.3
accD_p3	ACTCCCCGCTCTCTTGTTAT	ATTTTTCCCTACTGACCGCC	50.0	50.0	58.1	57.9	2283-2329 bp	1.0	83.1
accD_p4	ACTCCCCGCTCTCTTGTTAT	TGCAACCAACAATAACCCCA	50.0	45.0	58.1	57.9	2592-2641 bp	1.0	83.0
accD_p5	ACTCCCCGCTCTCTTGTTAT	TTCCCTACTGACCGCCATAT	50.0	50.0	58.1	57.9	2325 bp	0.5	58.2