Hotspot

rpl36-rps8

LSC · 78725-79206 · 482 bp

Back to Phaseolus

Pi 0.0355

MarkerSeek score 63.10

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 482
variable_sites: 17
pi: 0.0355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	GAAATCACCCGACCTCGTAC	TCGAATTTTAGGGGGCATGG	55.0	50.0	58.1	57.9	1184-1193 bp	1.0	82.0
rpl36-rps8_p2	GAAATCACCCGACCTCGTAC	TTACCATGGGGAAGGACACT	55.0	50.0	58.1	57.9	1479-1488 bp	1.0	81.7
rpl36-rps8_p3	CGAGACCAGGCCCTTTTATC	TTACCATGGGGAAGGACACT	55.0	50.0	58.0	57.9	1634 bp	0.5	59.2
rpl36-rps8_p4	CGAGACCAGGCCCTTTTATC	TCGAATTTTAGGGGGCATGG	55.0	50.0	58.0	57.9	1339 bp	0.5	59.1
rpl36-rps8_p5	CGAGACCAGGCCCTTTTATC	GGCATGGGGATTGTCATTCT	55.0	50.0	58.0	57.9	1326 bp	0.5	59.0