Hotspot

rps11-rpl36

LSC · 78284-78610 · 327 bp

Back to Phaseolus

Pi 0.0413

MarkerSeek score 72.80

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 327
variable_sites: 13
pi: 0.0413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TTCCGCGAACCAATACGTAC	ATTGATTCGAAGACGGGGAC	50.0	50.0	58.4	57.7	444-456 bp	1.0	79.0
rps11-rpl36_p2	CCGCGAACCAATACGTACAT	ATTGATTCGAAGACGGGGAC	50.0	50.0	58.4	57.7	442-454 bp	1.0	78.5
rps11-rpl36_p3	ATTCCGCGAACCAATACGTA	ATTGATTCGAAGACGGGGAC	45.0	50.0	57.4	57.7	445-457 bp	1.0	77.3
rps11-rpl36_p4	TTCCGCGAACCAATACGTAC	GATTGATTCGAAGACGGGGA	50.0	50.0	58.4	57.4	445-457 bp	1.0	76.7
rps11-rpl36_p5	GATTCCGCGAACCAATACGT	ATTGATTCGAAGACGGGGAC	50.0	50.0	58.7	57.7	446-458 bp	1.0	76.2