Hotspot

trnG-UCC-trnS-GCU

LSC · 54566-55185 · 620 bp

Back to Phaseolus

Pi 0.038

MarkerSeek score 71.70

Primer available yes

Label trnG-UCC-trnS-GCU

Feature Metadata

label_name: trnG-UCC-trnS-GCU
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 23
pi: 0.038
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnS-GCU_p1	TCCATGAAACGAATCGCACT	ATCCGACGCTATAGTCCACT	45.0	50.0	57.9	57.7	684-695 bp	1.0	81.4
trnG-UCC-trnS-GCU_p2	TCCATGAAACGAATCGCACT	GCAATCCGACGCTATAGTCC	45.0	55.0	57.9	58.3	687-698 bp	1.0	81.1
trnG-UCC-trnS-GCU_p3	GTCCATGAAACGAATCGCAC	ATCCGACGCTATAGTCCACT	50.0	50.0	57.8	57.7	685-696 bp	1.0	80.8
trnG-UCC-trnS-GCU_p4	GTCCATGAAACGAATCGCAC	GCAATCCGACGCTATAGTCC	50.0	55.0	57.8	58.3	688-699 bp	1.0	80.6
trnG-UCC-trnS-GCU_p5	TCCATGAAACGAATCGCACT	TCCGACGCTATAGTCCACTC	45.0	55.0	57.9	58.4	683-694 bp	1.0	80.3