Hotspot

rpoC1-rpoC2

LSC · 42634-42832 · 199 bp

Back to Phaseolus

Pi 0.0205

MarkerSeek score 66.20

Primer available yes

Label rpoC1-rpoC2

Feature Metadata

label_name: rpoC1-rpoC2
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 4
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoC2_p1	TTTTTGCCGGGCCTATTCAT	TGGCAGTTCCACCTATCACT	45.0	50.0	58.1	58.3	285-287 bp	1.0	78.4
rpoC1-rpoC2_p2	TTTTTGCCGGGCCTATTCAT	TTAGATTGGTCCGTTCTGCC	45.0	50.0	58.1	57.6	252-254 bp	1.0	78.2
rpoC1-rpoC2_p3	TTGCCGGGCCTATTCATATG	TGGCAGTTCCACCTATCACT	50.0	50.0	57.8	58.3	282-284 bp	1.0	77.9
rpoC1-rpoC2_p4	TTGCCGGGCCTATTCATATG	TTAGATTGGTCCGTTCTGCC	50.0	50.0	57.8	57.6	249-251 bp	1.0	77.8
rpoC1-rpoC2_p5	GCTGTACAAGGTTTTTGCCG	TGGCAGTTCCACCTATCACT	50.0	50.0	58.2	58.3	296-298 bp	1.0	77.5