Hotspot

psbD-trnT-GGU

LSC · 31192-32186 · 995 bp

Back to Phaseolus

Pi 0.0363
MarkerSeek score 73.00
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
995
variable_sites
36
pi
0.0363
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 CCGCAACCAGTCATCCATAA GTGGTAGAGTAACGCCATGG 50.0 55.0 58.0 58.1 1125-1143 bp 1.0 81.9
psbD-trnT-GGU_p2 CCGCAACCAGTCATCCATAA TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.0 58.1 1128-1146 bp 1.0 81.8
psbD-trnT-GGU_p3 CACAAAAACGAAACGGTCCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1146-1164 bp 1.0 80.8
psbD-trnT-GGU_p4 CCACAAAAACGAAACGGTCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1147-1165 bp 1.0 80.8
psbD-trnT-GGU_p5 CCCACAAAAACGAAACGGTC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1148-1166 bp 1.0 80.8