Hotspot

trnG-GCC-psbZ

LSC · 27218-27913 · 696 bp

Back to Phaseolus

Pi 0.0284
MarkerSeek score 69.80
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
696
variable_sites
19
pi
0.0284
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA CTTCTCCTGAAGGTTGGTCA 50.0 50.0 57.8 56.8 823-850 bp 1.0 73.2
trnG-GCC-psbZ_p2 CGTCTTCTCCTTGGCAAAGA CTTCTCCTGAAGGTTGGTCAA 50.0 47.6 57.8 57.5 823-850 bp 1.0 71.1
trnG-GCC-psbZ_p3 CGTCTTCTCCTTGGCAAAGA TCTCCTGAAGGTTGGTCAAA 50.0 45.0 57.8 56.3 821-848 bp 1.0 69.3
trnG-GCC-psbZ_p4 CGTCTTCTCCTTGGCAAAGA TTCTCCTGAAGGTTGGTCAA 50.0 45.0 57.8 56.3 822-849 bp 1.0 69.3
trnG-GCC-psbZ_p5 CGTCTTCTCCTTGGCAAAGA TTCTCCTGAAGGTTGGTCAAA 50.0 42.9 57.8 57.1 822-849 bp 1.0 67.5