Hotspot

rbcL

LSC · 57021-58463 · 1443 bp

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Pi 0.0139
MarkerSeek score 54.60
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1443
variable_sites
20
pi
0.0139
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 TGAGTTGTAGGGAGGGACTT TGCAATCAATTAGAAGAACAAAGA 50.0 29.2 57.3 55.3 1491-1494 bp 1.0 41.1
rbcL_p2 TCATGAGTTGTAGGGAGGGA TGCAATCAATTAGAAGAACAAAGA 50.0 29.2 57.1 55.3 1494-1497 bp 1.0 41.1
rbcL_p3 CATGAGTTGTAGGGAGGGAC TGCAATCAATTAGAAGAACAAAGA 55.0 29.2 57.0 55.3 1493-1496 bp 1.0 41.1
rbcL_p4 CGAGTAGACCCTGTTGTTGT TGCAATCAATTAGAAGAACAAAGA 50.0 29.2 57.2 55.3 1527-1530 bp 1.0 41.0
rbcL_p5 TGCAAAGGTTTCATTTACGCC TGCAATCAATTAGAAGAACAAAGA 42.9 29.2 58.0 55.3 1558-1561 bp 1.0 41.0