Hotspot

psbA

LSC · 101-1162 · 1062 bp

Back to Pharus

Pi 0.0132
MarkerSeek score 66.30
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
14
pi
0.0132
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 TCTGGCTTTCCTTCCTTCAA AATACGTGTGCTTGGGAGTC 45.0 50.0 56.7 57.9 1179-1180 bp 1.0 69.5
psbA_p2 TTCTGGCTTTCCTTCCTTCA AATACGTGTGCTTGGGAGTC 45.0 50.0 56.7 57.9 1180-1181 bp 1.0 69.5
psbA_p3 TCTGGCTTTCCTTCCTTCAAA AATACGTGTGCTTGGGAGTC 42.9 50.0 57.4 57.9 1179-1180 bp 1.0 67.5
psbA_p4 TTCTGGCTTTCCTTCCTTCAA AATACGTGTGCTTGGGAGTC 42.9 50.0 57.4 57.9 1180-1181 bp 1.0 67.5
psbA_p5 TTTCTGGCTTTCCTTCCTTCA AATACGTGTGCTTGGGAGTC 42.9 50.0 57.4 57.9 1181-1182 bp 1.0 67.5