Hotspot

ndhH-ndhF

SSC · 106291-106325 · 35 bp

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Pi 0.0323
MarkerSeek score 73.60
Primer available yes
Label ndhH-ndhF

Feature Metadata

label_name
ndhH-ndhF
feature_type
igs
region
SSC
length_bp
35
variable_sites
1
pi
0.0323
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-ndhF_p1 TGCACCAGAATTCTTGGAGA AAATATGTAGGGGGAGGGCG 45.0 55.0 56.7 58.9 152-166 bp 1.0 65.0
ndhH-ndhF_p2 TGCACCAGAATTCTTGGAGA GTAGGGGGAGGGCGAATAT 45.0 57.9 56.7 57.9 146-160 bp 1.0 63.9
ndhH-ndhF_p3 TGCACCAGAATTCTTGGAGA GTAGGGGGAGGGCGAATATC 45.0 60.0 56.7 59.1 146-160 bp 1.0 63.8
ndhH-ndhF_p4 TGCACCAGAATTCTTGGAGAA AAATATGTAGGGGGAGGGCG 42.9 55.0 57.5 58.9 152-166 bp 1.0 63.0
ndhH-ndhF_p5 GCAATAACCGTAAATGCACCA AAATATGTAGGGGGAGGGCG 42.9 55.0 57.5 58.9 166-180 bp 1.0 62.9