Hotspot

infA-rps8

LSC · 79100-79177 · 78 bp

Pi 0.0385

MarkerSeek score 73.90

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 78
variable_sites: 3
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	AGTGACTTTTGCTTCCCTCG	ATTTTGGGCGGAATGGGAAT	50.0	45.0	58.1	58.1	246-247 bp	1.0	79.1
infA-rps8_p2	ATCATTCTCTAGGCGAACGC	ATTTTGGGCGGAATGGGAAT	50.0	45.0	57.9	58.1	309-310 bp	1.0	78.7
infA-rps8_p3	GAACATTCCGTTGGGTAGGG	ATTTTGGGCGGAATGGGAAT	55.0	45.0	58.3	58.1	288-289 bp	1.0	77.8
infA-rps8_p4	GGAACATTCCGTTGGGTAGG	ATTTTGGGCGGAATGGGAAT	55.0	45.0	58.3	58.1	289-290 bp	1.0	77.8
infA-rps8_p5	CTTCCCTCGGGGTTCTTTTT	ATTTTGGGCGGAATGGGAAT	50.0	45.0	57.7	58.1	235-236 bp	1.0	77.7