Hotspot

atpI-atpH

LSC · 33425-34236 · 812 bp

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Pi 0.0296
MarkerSeek score 75.70
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
812
variable_sites
24
pi
0.0296
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 CGAATCCATGGAGGGTCATC GCAGTACCTTGACCAACTCC 55.0 55.0 57.8 58.2 929-930 bp 1.0 79.1
atpI-atpH_p2 CGAATCCATGGAGGGTCATC GCCAATCCAGCAGCAATAAC 55.0 50.0 57.8 57.8 884-885 bp 1.0 79.0
atpI-atpH_p3 CGAATCCATGGAGGGTCATC TCCAGCAGCAATAACGGAAG 55.0 50.0 57.8 58.3 879-880 bp 1.0 78.7
atpI-atpH_p4 CGAATCCATGGAGGGTCATC CCTTCTACAGCTTGTCCTGC 55.0 55.0 57.8 58.3 950-951 bp 1.0 78.5
atpI-atpH_p5 CGAATCCATGGAGGGTCATC ATCCAGCAGCAATAACGGAA 55.0 45.0 57.8 57.6 880-881 bp 1.0 77.4