Hotspot

psbM-petN

LSC · 18075-19244 · 1170 bp

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Pi 0.0257
MarkerSeek score 65.40
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
1170
variable_sites
30
pi
0.0257
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 TGCTACTGCACTGTTCATTCT TAGTAAGTCTCGCTTGGGCT 42.9 50.0 57.6 58.2 1332-1339 bp 1.0 69.4
psbM-petN_p2 TGCTACTGCACTGTTCATTCT GTAGTATGGGGGAGGAGTGG 42.9 60.0 57.6 58.3 1276-1283 bp 1.0 68.5
psbM-petN_p3 TGCTACTGCACTGTTCATTCT TAGTATGGGGGAGGAGTGGA 42.9 55.0 57.6 58.4 1275-1282 bp 1.0 67.8
psbM-petN_p4 TGCTACTGCACTGTTCATTC TAGTAAGTCTCGCTTGGGCT 45.0 50.0 56.1 58.2 1332-1339 bp 1.0 65.3
psbM-petN_p5 TGCTACTGCACTGTTCATTCT AGTAGTATGGGGGAGGAGTG 42.9 55.0 57.6 57.2 1277-1284 bp 1.0 64.5