Hotspot

clpP

LSC · 72254-74265 · 2012 bp

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Pi 0.0447

MarkerSeek score 80.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2012
variable_sites: 360
pi: 0.0447
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGCACCAACCGAAAACCATA	GGGGGTGGAGAGTTGATACT	45.0	55.0	58.0	58.1	2896 bp	0.076923	42.0
clpP_p2	CTGAACGACTCGCACATACA	GGGGGTGGAGAGTTGATACT	50.0	55.0	58.0	58.1	2721 bp	0.076923	41.9
clpP_p3	ACATGATCTGAACGACTCGC	GGGGGTGGAGAGTTGATACT	50.0	55.0	58.1	58.1	2728 bp	0.076923	41.4
clpP_p4	ATGATCTGAACGACTCGCAC	GGGGGTGGAGAGTTGATACT	50.0	55.0	58.1	58.1	2726 bp	0.076923	41.4
clpP_p5	GATCTGAACGACTCGCACAT	GGGGGTGGAGAGTTGATACT	50.0	55.0	58.1	58.1	2724 bp	0.076923	41.4