Hotspot

ndhF-rpl32

SSC · 114791-115835 · 1045 bp

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Pi 0.0296

MarkerSeek score 76.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1045
variable_sites: 121
pi: 0.0296
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGCAACTGAACCGGCAAATA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	2172-2516 bp	1.0	90.1
ndhF-rpl32_p2	ATTTAGCAACTGAACCGGCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	2176-2520 bp	1.0	90.1
ndhF-rpl32_p3	TAGCAACTGAACCGGCAAAT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	2173-2517 bp	1.0	90.1
ndhF-rpl32_p4	TAGGGCGTGTGAACCAAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1963-2307 bp	0.923077	87.6
ndhF-rpl32_p5	TAGGGCGTGTGAACCAAAAA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1964-2308 bp	0.923077	87.6