Hotspot

trnI-CAU-ycf2

IRb · 89625-89959 · 335 bp

Back to Phanera

Pi 0.0766

MarkerSeek score 76.90

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 335
variable_sites: 89
pi: 0.0766
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	AATCATGGTCCTTGCGGATC	50.0	50.0	58.0	58.0	1026-2107 bp	0.923077	87.5
trnI-CAU-ycf2_p2	CTTATTGGAGGGTCCCGTTG	AATCATGGTCCTTGCGGATC	55.0	50.0	58.0	58.0	1091-2172 bp	0.923077	87.0
trnI-CAU-ycf2_p3	TAGAACAACCCGGTTGAAGC	AATCATGGTCCTTGCGGATC	50.0	50.0	58.1	58.0	1528-2572 bp	0.769231	79.3
trnI-CAU-ycf2_p4	AGAAGAGTTCGACCCATTGC	AATCATGGTCCTTGCGGATC	50.0	50.0	57.9	58.0	1409 bp	0.076923	42.1
trnI-CAU-ycf2_p5	GAAGAGTTCGACCCATTGCT	AATCATGGTCCTTGCGGATC	50.0	50.0	57.9	58.0	1408 bp	0.076923	42.1