Hotspot

rps3-rps19

LSC · 86165-87112 · 948 bp

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Pi 0.0263

MarkerSeek score 77.40

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 948
variable_sites: 104
pi: 0.0263
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	GCCTACTCGAAACTTCCTGG	50.0	55.0	57.5	58.0	978-1266 bp	0.846154	81.4
rps3-rps19_p2	TACCAAGTCTGAAACCGAGC	TTCGCCTACTCGAAACTTCC	50.0	50.0	57.5	57.6	981-1269 bp	0.846154	78.6
rps3-rps19_p3	TACCAAGTCTGAAACCGAGC	CGCCTACTCGAAACTTCCTG	50.0	55.0	57.5	58.4	979-1267 bp	0.846154	78.5
rps3-rps19_p4	TACCAAGTCTGAAACCGAGC	TCGCCTACTCGAAACTTCCT	50.0	50.0	57.5	58.5	980-1268 bp	0.846154	77.8
rps3-rps19_p5	TACCAAGTCTGAAACCGAGC	AACTTCCTGGGACATGATGC	50.0	50.0	57.5	57.9	968-1256 bp	0.769231	77.1