Hotspot

rbcL-accD

LSC · 59242-60960 · 1719 bp

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Pi 0.0498

MarkerSeek score 78.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1719
variable_sites: 267
pi: 0.0498
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	CTCGATCAACCGGGTGATTT	50.0	50.0	58.0	58.0	1957-2957 bp	1.0	91.5
rbcL-accD_p2	AGTCCTGAATTAGCTGCTGC	CGGAAGCACACACGAGAATA	50.0	50.0	58.0	58.0	2003-2787 bp	0.923077	88.6
rbcL-accD_p3	AGATCTTTGGCGACGATTCC	CGGAAGCACACACGAGAATA	50.0	50.0	58.1	58.0	2182-2966 bp	0.923077	88.4
rbcL-accD_p4	AGTCCTGAATTAGCTGCTGC	GATTCCTCCTCCTCCTCCTC	50.0	60.0	58.0	58.0	2285 bp	0.076923	42.7
rbcL-accD_p5	AGATCTTTGGCGACGATTCC	GATTCCTCCTCCTCCTCCTC	50.0	60.0	58.1	58.0	2464 bp	0.076923	42.5