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psbZ-trnG-GCC

LSC · 38514-39105 · 592 bp

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Pi 0.0203

MarkerSeek score 77.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 53
pi: 0.0203
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTGTTGTATTTGCTTCGCCG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	737-786 bp	1.0	87.3
psbZ-trnG-GCC_p2	TTGTATTTGCTTCGCCGGAT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	58.3	57.8	734-783 bp	1.0	86.3
psbZ-trnG-GCC_p3	CTGTTGTATTTGCTTCGCCG	AAAAAGACGGAGGCGGATAG	50.0	50.0	58.1	57.7	772-821 bp	1.0	86.0
psbZ-trnG-GCC_p4	CGGATGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.4	57.8	719-768 bp	1.0	85.4
psbZ-trnG-GCC_p5	CCTGTTGTATTTGCTTCGCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	738-787 bp	0.923077	82.7