Hotspot

atpH-atpI

LSC · 14432-15147 · 716 bp

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Pi 0.0149

MarkerSeek score 77.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 54
pi: 0.0149
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCAGCGGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	757-851 bp	1.0	87.9
atpH-atpI_p2	ATAACGGAAGCAGCGGAAAT	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	804-898 bp	1.0	87.8
atpH-atpI_p3	TCCCAGGTCCAATAGAAGCA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	801-895 bp	1.0	87.7
atpH-atpI_p4	TCCCAGGTCCAATAGAAGCA	CCCGTCATGTTCCTTGGATT	50.0	50.0	58.0	57.9	848-942 bp	1.0	87.6
atpH-atpI_p5	TACCTTGACCAATCCCAGGT	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.9	57.9	860-954 bp	1.0	87.4