Hotspot

trnS-GCU-trnG-UCC

LSC · 8738-9282 · 545 bp

Back to Phanera

Pi 0.0364

MarkerSeek score 77.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 545
variable_sites: 78
pi: 0.0364
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	AGTCCACTCAGCCATCTCTC	GGGGTCCATAGAACGAATCG	55.0	55.0	58.5	57.8	290-625 bp	1.0	86.7
trnS-GCU-trnG-UCC_p2	AGTCCACTCAGCCATCTCTC	GGTCCATAGAACGAATCGCA	55.0	50.0	58.5	57.8	288-623 bp	1.0	86.1
trnS-GCU-trnG-UCC_p3	TAGTCCACTCAGCCATCTCT	GGGGTCCATAGAACGAATCG	50.0	55.0	57.2	57.8	291-626 bp	1.0	84.1
trnS-GCU-trnG-UCC_p4	AGTCCACTCAGCCATCTCTC	GGGTCCATAGAACGAATCGC	55.0	55.0	58.5	58.5	289-624 bp	1.0	83.9
trnS-GCU-trnG-UCC_p5	TAGTCCACTCAGCCATCTCT	GGTCCATAGAACGAATCGCA	50.0	50.0	57.2	57.8	289-624 bp	1.0	83.6