Hotspot

rps16-trnQ-UUG

LSC · 6621-7270 · 650 bp

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Pi 0.0294

MarkerSeek score 80.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 71
pi: 0.0294
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCACACGTTGCTTTCTACCC	AAGGTTCGAATCCTTCCGTC	50.0	50.0	58.1	57.6	293-728 bp	0.923077	84.5
rps16-trnQ-UUG_p2	TCACACGTTGCTTTCTACCC	GGTCCCGTTATTCGAAGGTT	50.0	50.0	58.1	57.6	307-742 bp	0.923077	84.3
rps16-trnQ-UUG_p3	ACACGTTGCTTTCTACCCAA	AAGGTTCGAATCCTTCCGTC	45.0	50.0	57.7	57.6	291-726 bp	0.923077	82.8
rps16-trnQ-UUG_p4	TCACACGTTGCTTTCTACCC	TTGGTCCCGTTATTCGAAGG	50.0	50.0	58.1	57.6	309-744 bp	0.846154	80.7
rps16-trnQ-UUG_p5	ACACGTTGCTTTCTACCCAA	TTGGTCCCGTTATTCGAAGG	45.0	50.0	57.7	57.6	307-742 bp	0.846154	79.1