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clpP-psbB

LSC · 67625-68138 · 514 bp

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Pi 0.0137

MarkerSeek score 65.60

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 514
variable_sites: 7
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCCAAGTCGCTTCTTCATC	TATAAAGCCATCGAGCCAGC	50.0	50.0	57.9	57.8	692-695 bp	1.0	79.1
clpP-psbB_p2	ACCCAAGTCGCTTCTTCATC	CCAGCCCAACCAGAAACTAG	50.0	55.0	57.9	58.2	677-680 bp	1.0	79.1
clpP-psbB_p3	TCAACCCAAGTCGCTTCTTC	TATAAAGCCATCGAGCCAGC	50.0	50.0	58.1	57.8	695-698 bp	1.0	78.9
clpP-psbB_p4	TCAACCCAAGTCGCTTCTTC	CCAGCCCAACCAGAAACTAG	50.0	55.0	58.1	58.2	680-683 bp	1.0	78.9
clpP-psbB_p5	ACCCAAGTCGCTTCTTCATC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	593-596 bp	1.0	78.8