Hotspot

rpl33-rps18

LSC · 64731-65001 · 271 bp

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Pi 0.0111

MarkerSeek score 46.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 271
variable_sites: 3
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGCAAGCATACGACTCATCA	GAAAAGGTTGTTTAGATGTATACA	50.0	29.2	58.4	53.1	332-340 bp	1.0	41.0
rpl33-rps18_p2	TCGCAAGCATACGACTCATC	GAAAAGGTTGTTTAGATGTATACA	50.0	29.2	58.2	53.1	333-341 bp	1.0	40.9
rpl33-rps18_p3	TGTCGTTATTGTCGCAAGCA	GAAAAGGTTGTTTAGATGTATACA	45.0	29.2	58.5	53.1	344-352 bp	1.0	40.9
rpl33-rps18_p4	TTGTCGTTATTGTCGCAAGC	GAAAAGGTTGTTTAGATGTATACA	45.0	29.2	57.4	53.1	345-353 bp	1.0	40.9
rpl33-rps18_p5	TCGCAAGCATACGACTCATC	GAAAAGGTTGTTTAGATGTATACAT	50.0	28.0	58.2	53.6	333-341 bp	1.0	40.9