Hotspot

ycf4-cemA

LSC · 58204-58445 · 242 bp

Back to Phalaris

Pi 0.0373

MarkerSeek score 74.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 242
variable_sites: 9
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	CCCCAGGGCAAAAATACGAT	45.0	50.0	57.9	58.2	333-430 bp	1.0	79.6
ycf4-cemA_p2	CTTCTTGCGCGTACCAATTG	CCCCAGGGCAAAAATACGAT	50.0	50.0	58.1	58.2	338-435 bp	1.0	79.3
ycf4-cemA_p3	CTTGCGCGTACCAATTGAAG	CCCCAGGGCAAAAATACGAT	50.0	50.0	58.1	58.2	335-432 bp	1.0	79.3
ycf4-cemA_p4	ACAAAAAGCTGCCGAATTGG	CCCCAGGGCAAAAATACGAT	45.0	50.0	57.8	58.2	362-459 bp	1.0	78.9
ycf4-cemA_p5	CTACTTCTTGCGCGTACCAA	CCCCAGGGCAAAAATACGAT	50.0	50.0	58.3	58.2	341-438 bp	1.0	77.9