Hotspot

psaA-ycf3

LSC · 40936-41577 · 642 bp

Back to Phalaris

Pi 0.0128

MarkerSeek score 67.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 642
variable_sites: 8
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGTCTGGCCCATTCTTCAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	786-804 bp	1.0	81.9
psaA-ycf3_p2	GGTCTGGCCCATTCTTCAAA	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.1	58.1	782-800 bp	1.0	81.6
psaA-ycf3_p3	GGTCTGGCCCATTCTTCAAA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.1	57.7	821-839 bp	1.0	79.7
psaA-ycf3_p4	TTTAACTTCTGGTTCCGGCG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	733-751 bp	1.0	78.8
psaA-ycf3_p5	TGTTCTTGAGAAATGGCCGG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	805-823 bp	1.0	78.7